RNase III regulates actinomycin production in Streptomyces antibioticus 公开

Abstract

Streptomyces plays an important role in the production of antibiotics; almost two- thirds of all known natural antibiotics come from the secondary metabolites of Streptomyces. Using genetic modifications, production of these secondary metabolites can be manipulated. Previous studies have shown that the absB locus of Streptomyces coelicolor encodes RNase III, which regulates the production of antibiotics in S. coelicolor. The absB locus can also be found in Streptomyces antibioticus, which led to the hypothesis that RNase III regulates the production of antibiotics in S. antibioticus, just like in S. coelicolor. To test this hypothesis, the RNase III gene in the chromosome of S. antibioticus was disrupted through the use of disruption plasmids, which contained a truncated fragment of the RNase III gene. Once the RNase III gene was disrupted in the chromosome of S. antibioticus, the production of the antibiotic, actinomycin D, by the resulting mutants was measured. This study showed that actinomycin production by the RNase III gene disrupted mutants was significantly reduced. The result indicated that RNase III regulates the production of antibiotics in S. antibioticus, just like in S. coelicolor. This finding also supported the idea that RNase III globally regulates the production of antibiotics in Streptomyces in general

Table of Contents

Purpose - 1
Introduction - 1
Materials and Methods - 5
Results - 9
Discussions - 12
Tables and Figures - 16
Table 1 - 16
Table 2 - 17
Figure 1 - 18
Figure 2 - 19
Figure 3 - 20
Figure 4 - 21
Figure 5 - 22
Figure 6 - 23
Figure 7 - 24
Figure 8 - 25
Figure 9 - 26
Figure 10 - 27
Figure 11 - 28
Figure 12 - 29
Figure 13 - 30
References - 31

About this Honors Thesis

Rights statement

• Permission granted by the author to include this thesis or dissertation in this repository. All rights reserved by the author. Please contact the author for information regarding the reproduction and use of this thesis or dissertation.

School	Emory College
Department	Biology
Degree	BS
Submission	Honors Thesis
Language	English
Research Field	Biology, Microbiology
关键词	RNase III Streptomyces antibioticus antibiotic production
Committee Chair / Thesis Advisor	Jones, George H, Emory University
Committee Members	Campbell, Kathleen, Emory University Nickerson, John, Emory University

最新修改

Primary PDF

Thumbnail	Title	Date Uploaded	Actions
	RNase III regulates actinomycin production in Streptomyces antibioticus ()	2018-08-28 13:06:39 -0400	Press to Select an action Download

Supplemental Files

Thumbnail	Title	Date Uploaded	Actions