Delayed Treadmill Training and Synaptic Stripping of Axotomized Motoneurons 公开
Wilson, Natalee Kay (2011)
Abstract
Delayed Treadmill Training and Synaptic Stripping of Axotomized
Motoneurons
By Natalee Kay Wilson
When a peripheral nerve is injured, sensory and motor axons in the
periphery are disconnected from the central nervous system (CNS).
In addition, synaptic inputs withdraw from the somata and proximal
dendrites of the injured motoneuron, a process known as synaptic
stripping. After moderate daily treadmill training, the expected
loss of synaptic inputs onto axotomized motoneurons due to synaptic
stripping is not observed. Whether this effect of treadmill
training is due to the prevention of the withdrawal of synaptic
inputs or a restoration of synapses is not clear. In this study,
the onset of treadmill training was delayed until one week
following peripheral nerve transection. At that time, substantial
withdrawal of synapses from the axotomized motoneurons had
occurred. The ability of treadmill training to restore synapses
onto these motoneurons was evaluated. Synaptic coverage was assayed
by measuring the proportion of somata and proximal dendrites of
retrogradely labeled motoneurons covered by terminals
immunopositive for markers of excitatory VGLUT1 and inhibitory
GAD67 synapses. The coverage of axotomized motoneurons by terminals
containing VGLUT1 in delayed trained mice was similar to that found
in intact controls, suggesting a restoration of VGLUT1+ boutons
onto the somata and proximal dendrites of the motoneuron. The
expected loss of GAD67+ boutons that was observed in axotomized
motoneurons in untrained mice was not found in trained mice,
strongly suggesting that the delayed treadmill training had a
restorative effect. Thus, if treadmill training is delayed by one
week after nerve transection, the anticipated loss of synaptic
coverage on the axotomized motoneurons does not occur. This is
evidence for treadmill training having a restorative effect on the
stripped synapses.
Delayed Treadmill Training and Synaptic Stripping of Axotomized
Motoneurons
By
Natalee Kay Wilson
B.S., Emory University, Biology
Advisor: Arthur English, Ph.D.
A thesis submitted to the Faculty of the
James T. Laney School of Graduate Studies of Emory University
in partial fulfillment of the requirements for the degree of
Master of Science
in Biology
2011
Table of Contents
Table of Contents
I. Introduction 1
II. Methods 7
Animals 7
Muscle Injections/Retrograde Labeling 7
Surgical Methods 7
Treadmill Training 8
Immunohistochemistry 8
Image Analysis 9
Statistical Analysis 10
III. Results 11
Effect of delayed treadmill training on synaptic coverage by
VGLUT1+ terminals 11
Effect of delayed treadmill training on synaptic coverage by GAD67+ terminals 13
IV. Discussion 15
V. Tables 18
Table 1. Groups of mice used in this study 18
VI. References 19
VII. Figures 23
Figure 1. Retrogradely labeled motoneurons (red) with synaptic
boutons immunopostive for VGLUT1 (blue) and GAD67 (green). 23
Figure 2. Retrogradely labeled motoneuron with GAD67 and Profile
plot 24
Figure 3. Effect of delayed treadmill training on VGLUT1+ synapses
25
About this Master's Thesis
School | |
---|---|
Department | |
Degree | |
Submission | |
Language |
|
Research Field | |
关键词 | |
Committee Chair / Thesis Advisor | |
Committee Members |
Primary PDF
Thumbnail | Title | Date Uploaded | Actions |
---|---|---|---|
Delayed Treadmill Training and Synaptic Stripping of Axotomized Motoneurons () | 2018-08-28 14:45:40 -0400 |
|
Supplemental Files
Thumbnail | Title | Date Uploaded | Actions |
---|