Characterization of Kell Glycoprotein Transgenic Mice: Murine Model for Immune Response to Transfusion Público

Henry, Kate (2011)

Permanent URL: https://etd.library.emory.edu/concern/etds/nc580n484?locale=es
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Abstract


Abstract
Characterization of Kell Glycoprotein Transgenic Mice: Murine Model for Immune Response to
Transfusion
By Kate Henry
Mouse models are useful tools to study the generation and effects of immunity to alloantigens on
transfused and transplanted tissues. However, mouse models are much more relevant to the
particulars of human biology when they express the human form of the alloantigens of interest. Our
laboratory generated two strains of transgenic mice, each of which expresses one of two antithetical
antigens (Cellano and Kell epitopes) from the Kell glycoprotein blood group system that differ by a
single amino acid (Methionine-Threonine at position 193). The Kell glycoprotein red blood cell
antigen is one of the most clinically significant antigens in blood transfusions after ABO and RhD
red blood cell antigens. These Kell glycoprotein transgenic mice, named Cellano or Kell based on
the variant that they express, are useful models to study immune responses to transfusion. Here we
describe our initial characterization of these Kell glycoprotein transgenic mice.


Characterization of Kell Glycoprotein Transgenic Mice: Murine Model for Immune Response to
Transfusion
By
Kate Henry
B.S. in Biology, Brandeis University, 2009
Advisor: James Zimring, Ph.D., M.D.
A thesis submitted to the Faculty of the
James T. Laney School of Graduate Studies of Emory University
in partial fulfillment of the requirements for the degree of
Master of Science
in Graduate Division of Biological and Biomedical Science,
Immunology and Molecular Pathogenesis
2011

Table of Contents



Table of Contents:
1. Introduction………………………………… .1-4
2. Methods……………………………………... 4-10
3. Results……………………………………….10-15
4. Discussion…………………………………...15-21
5. Figure 1……………………………………......22
6. Figure 2……………………………………...23-24

7. Figure 3……………………………………...25-26
8. Figure 4……………………………………….27
9. Figure 5……………………………………….28
10. Figure 6……………………………………...29
11. Figure 7……………………………………...30
12. Figure 8……………………………………...31
13. Figure 9……………………………………...32
14. Figure 10…………………………………….33
15. Figure 11…………………………………….34
16: Figure 12…………………………………….35
17. References………………………………….36-37


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