Characterization of Kell Glycoprotein Transgenic Mice: Murine Model for Immune Response to Transfusion Öffentlichkeit

Abstract

Abstract
Characterization of Kell Glycoprotein Transgenic Mice: Murine Model for Immune Response to
Transfusion
By Kate Henry
Mouse models are useful tools to study the generation and effects of immunity to alloantigens on
transfused and transplanted tissues. However, mouse models are much more relevant to the
particulars of human biology when they express the human form of the alloantigens of interest. Our
laboratory generated two strains of transgenic mice, each of which expresses one of two antithetical
antigens (Cellano and Kell epitopes) from the Kell glycoprotein blood group system that differ by a
single amino acid (Methionine-Threonine at position 193). The Kell glycoprotein red blood cell
antigen is one of the most clinically significant antigens in blood transfusions after ABO and RhD
red blood cell antigens. These Kell glycoprotein transgenic mice, named Cellano or Kell based on
the variant that they express, are useful models to study immune responses to transfusion. Here we
describe our initial characterization of these Kell glycoprotein transgenic mice.

Characterization of Kell Glycoprotein Transgenic Mice: Murine Model for Immune Response to
Transfusion
By
Kate Henry
B.S. in Biology, Brandeis University, 2009
Advisor: James Zimring, Ph.D., M.D.
A thesis submitted to the Faculty of the
James T. Laney School of Graduate Studies of Emory University
in partial fulfillment of the requirements for the degree of
Master of Science
in Graduate Division of Biological and Biomedical Science,
Immunology and Molecular Pathogenesis
2011

Table of Contents

Table of Contents:
1. Introduction………………………………… .1-4
2. Methods……………………………………... 4-10
3. Results……………………………………….10-15
4. Discussion…………………………………...15-21
5. Figure 1……………………………………......22
6. Figure 2……………………………………...23-24

7. Figure 3……………………………………...25-26
8. Figure 4……………………………………….27
9. Figure 5……………………………………….28
10. Figure 6……………………………………...29
11. Figure 7……………………………………...30
12. Figure 8……………………………………...31
13. Figure 9……………………………………...32
14. Figure 10…………………………………….33
15. Figure 11…………………………………….34
16: Figure 12…………………………………….35
17. References………………………………….36-37

About this Master's Thesis

Rights statement

• Permission granted by the author to include this thesis or dissertation in this repository. All rights reserved by the author. Please contact the author for information regarding the reproduction and use of this thesis or dissertation.

School	Laney Graduate School
Department	Biological and Biomedical Sciences
Degree	MS
Submission	Master's Thesis
Language	English
Research Field	Health Sciences, Immunology Biology, General
Stichwort	Novel Murine Model for Blood Transfusion
Committee Chair / Thesis Advisor	Zimring, James C, Emory University
Committee Members	Lukacher, Aron E, Emory University Evavold, Brian, Emory University Selvaraj, Periasamy, Emory University Ford, Mandy L, Emory University

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