The Effect of Electrical Field Exposure on F98 Rat Glioma-derived Spheroids in-vitro Restricted; Files Only
Rama-Krishnan, Arun (Spring 2024)
Abstract
The treatment of brain and central nervous system cancers remain incredibly complex due to challenges like the invasive nature of tumors and risky surgeries, emphasizing the critical need for innovative interventions to improve patient outcomes. Despite being the most common malignant brain tumor, Glioblastoma, a stage 4 glioma, has very grim statistics with only a 6.9% 5-year survival rate and average length of survival of 8 months. Electrotaxis, or the guided migration of cells via low voltage, direct current electrical fields present a potential method to move tumor cells to more treatable areas. In this study, we investigated the efficacy of continuous electrical fields to induce electrotaxis as well as its effect on Glioblastoma cell spheroidal aggregate size. We investigated this in-vitro using F98 rat-glioma-derived spheroidal cell aggregates. Spheroids were plated in a Matrigel-cell media solution within 8-well slides and subjected to an electrical field using a novel device that generated 250 V/m for a total of 3 hours with 30-minute intervals. Our data did not indicate any significant movement in any direction; however, spheroids did significantly decrease in size, relative to controls. Furthermore, in analyzing data from the 30 minutes to 1 hour subinterval, we found significant negative anodal frontier shift and cathodal bias, relative to controls. Overall, the findings of our study suggest that continuous exposure to 250 V/m direct current electrical fields can decrease the size of spheroidal aggregates containing F98 glioma cells. In the future, experimental repeats and experiments with different field parameters need to be investigated.
Table of Contents
Introduction
Background and History of GBM Treatment Options Electrotaxis as a Potential New Therapy Purpose and Rationale HypothesisMaterials and Methods
Experimental Protocol Cell Line and Culture Spheroid Generation Spheroid Plating Electrical Field Delivering Device and Setup Microscopy and Spheroid Analysis Statistical AnalysisResults
Spheroid Migration Results Spheroid Size ResultsDiscussion
Summary of Findings Limitations Implications and Future DirectionsSupplemental Figures
Works Cited
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File download under embargo until 24 November 2026 | 2024-04-10 09:03:01 -0400 | File download under embargo until 24 November 2026 |
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