The Effect of Electrical Field Exposure on F98 Rat Glioma-derived Spheroids in-vitro Restricted; Files Only

Abstract

The treatment of brain and central nervous system cancers remain incredibly complex due to challenges like the invasive nature of tumors and risky surgeries, emphasizing the critical need for innovative interventions to improve patient outcomes. Despite being the most common malignant brain tumor, Glioblastoma, a stage 4 glioma, has very grim statistics with only a 6.9% 5-year survival rate and average length of survival of 8 months. Electrotaxis, or the guided migration of cells via low voltage, direct current electrical fields present a potential method to move tumor cells to more treatable areas. In this study, we investigated the efficacy of continuous electrical fields to induce electrotaxis as well as its effect on Glioblastoma cell spheroidal aggregate size. We investigated this in-vitro using F98 rat-glioma-derived spheroidal cell aggregates. Spheroids were plated in a Matrigel-cell media solution within 8-well slides and subjected to an electrical field using a novel device that generated 250 V/m for a total of 3 hours with 30-minute intervals. Our data did not indicate any significant movement in any direction; however, spheroids did significantly decrease in size, relative to controls. Furthermore, in analyzing data from the 30 minutes to 1 hour subinterval, we found significant negative anodal frontier shift and cathodal bias, relative to controls. Overall, the findings of our study suggest that continuous exposure to 250 V/m direct current electrical fields can decrease the size of spheroidal aggregates containing F98 glioma cells. In the future, experimental repeats and experiments with different field parameters need to be investigated.

Table of Contents

Introduction                                                                                                                                    

Background and History of GBM                                                                                         Treatment Options                                                                                                                 Electrotaxis as a Potential New Therapy                                                                               Purpose and Rationale                                                                                                         Hypothesis                                                                                                                            

Materials and Methods                                                                                                                  

Experimental Protocol                                                                                                           Cell Line and Culture                                                                                                           Spheroid Generation                                                                                                             Spheroid Plating                                                                                                                  Electrical Field Delivering Device and Setup                                                                        Microscopy and Spheroid Analysis                                                                                     Statistical Analysis                                                                                                             

Results                                                                                                                                            

Spheroid Migration Results                                                                                                 Spheroid Size Results                                                                                                         

Discussion                                                                                                                                      

Summary of Findings                                                                                                          Limitations                                                                                                                           Implications and Future Directions                                                                                    

Supplemental Figures                                                                                                                  

Works Cited

About this Honors Thesis

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School	Emory College
Department	Neuroscience and Behavioral Biology
Degree	B.S.
Submission	Honors Thesis
Language	English
Research Field	Engineering, Electronics and Electrical Biology, Neuroscience
Keyword	Brain Cancer
Committee Chair / Thesis Advisor	Nassir Mokarram , Emory University
Committee Members	J. Alex Grizzell, Emory University Robert Wyttenbach, Emory University

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