Intensive Care Unit Acquired Weakness genes exist between non-survivors and survivors in patients with sepsis Public

Abstract

Sepsis is a potentially lethal condition, and intensive care unit (ICU) acquired weakness (ICUAW) is one of the complications of sepsis. Bioenergy failure is a plausible mechanism of organ failure and ICUAW, but the link between sepsis-related mortality and ICUAW remains unclear. Elucidating this biological link is crucial to the understanding of the pathophysiology of sepsis-related mortality. Using pre-identified ICUAW genes, a publicly available gene expression profile GSE54514 containing whole blood samples within 24 hours of sepsis onset was analyzed. Differential gene expression analysis identified 38 genes significantly different between 8 non-survivors and 13 survivors with primary bacteremia- and respiratory-triggered sepsis. Functional enrichment analysis of differentially expressed ICUAW genes identified impaired cadherin binding, sarcomere formation, and energy metabolism among non-survivors. Further, we interrogated ICUAW genes in patients with respiratory-triggered sepsis. In this population, a deficit in energy metabolism, cadherin binding, sarcomere formation, and granule secretion was observed. Our findings demonstrated an association between ICUAW genes and sepsis-related mortality in the early phase of sepsis. Finally, defects in energy metabolism and muscle fiber formation are likely resulting in septic patients who are non-survivors, especially in respiratory-triggered sepsis.

Table of Contents

Introduction 1

Materials and methods 2

Results 4

Discussion 5

Reference 8

Table 1 11

Figures 12

About this Master's Thesis

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School	Rollins School of Public Health
Department	Epidemiology
Subfield / Discipline	Epidemiology - MPH & MSPH
Degree	M.P.H.
Submission	Master's Thesis
Language	English
Research Field	Health Sciences, Epidemiology Biology, Bioinformatics Health Sciences, General
Mot-clé	Post Intensive Care Syndrome Sepsis Persistent Inflammation, Immunosuppression, and Catabolism Syndrome Intensive Care Unit Acquired Weakness Respiratory triggered sepsis
Committee Chair / Thesis Advisor	Sun, Yan, Emory University
Committee Members	Kamaleswaran, Rishikesan, Emory University

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	Supplementary information (This supplementary information includes study population characteristics and supplementary results.)	2022-04-13 11:39:37 -0400	Press to Select an action Download