Intensive Care Unit Acquired Weakness genes exist between non-survivors and survivors in patients with sepsis Open Access

Abstract

Sepsis is a potentially lethal condition, and intensive care unit (ICU) acquired weakness (ICUAW) is one of the complications of sepsis. Bioenergy failure is a plausible mechanism of organ failure and ICUAW, but the link between sepsis-related mortality and ICUAW remains unclear. Elucidating this biological link is crucial to the understanding of the pathophysiology of sepsis-related mortality. Using pre-identified ICUAW genes, a publicly available gene expression profile GSE54514 containing whole blood samples within 24 hours of sepsis onset was analyzed. Differential gene expression analysis identified 38 genes significantly different between 8 non-survivors and 13 survivors with primary bacteremia- and respiratory-triggered sepsis. Functional enrichment analysis of differentially expressed ICUAW genes identified impaired cadherin binding, sarcomere formation, and energy metabolism among non-survivors. Further, we interrogated ICUAW genes in patients with respiratory-triggered sepsis. In this population, a deficit in energy metabolism, cadherin binding, sarcomere formation, and granule secretion was observed. Our findings demonstrated an association between ICUAW genes and sepsis-related mortality in the early phase of sepsis. Finally, defects in energy metabolism and muscle fiber formation are likely resulting in septic patients who are non-survivors, especially in respiratory-triggered sepsis.

Table of Contents

Introduction 1

Materials and methods 2

Results 4

Discussion 5

Reference 8

Table 1 11

Figures 12

About this Master's Thesis

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School	Rollins School of Public Health
Department	Epidemiology
Subfield / Discipline	Epidemiology - MPH & MSPH
Degree	M.P.H.
Submission	Master's Thesis
Language	English
Research Field	Health Sciences, Epidemiology Biology, Bioinformatics Health Sciences, General
Keyword	Post Intensive Care Syndrome Sepsis Persistent Inflammation, Immunosuppression, and Catabolism Syndrome Intensive Care Unit Acquired Weakness Respiratory triggered sepsis
Committee Chair / Thesis Advisor	Sun, Yan, Emory University
Committee Members	Kamaleswaran, Rishikesan, Emory University

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	Supplementary information (This supplementary information includes study population characteristics and supplementary results.)	2022-04-13 11:39:37 -0400	Press to Select an action Download