Intensive Care Unit Acquired Weakness genes exist between non-survivors and survivors in patients with sepsis Open Access

Kobara, Seibi (Spring 2022)

Permanent URL: https://etd.library.emory.edu/concern/etds/4m90dw84s?locale=en
Published

Abstract

Sepsis is a potentially lethal condition, and intensive care unit (ICU) acquired weakness (ICUAW) is one of the complications of sepsis. Bioenergy failure is a plausible mechanism of organ failure and ICUAW, but the link between sepsis-related mortality and ICUAW remains unclear. Elucidating this biological link is crucial to the understanding of the pathophysiology of sepsis-related mortality. Using pre-identified ICUAW genes, a publicly available gene expression profile GSE54514 containing whole blood samples within 24 hours of sepsis onset was analyzed. Differential gene expression analysis identified 38 genes significantly different between 8 non-survivors and 13 survivors with primary bacteremia- and respiratory-triggered sepsis. Functional enrichment analysis of differentially expressed ICUAW genes identified impaired cadherin binding, sarcomere formation, and energy metabolism among non-survivors. Further, we interrogated ICUAW genes in patients with respiratory-triggered sepsis. In this population, a deficit in energy metabolism, cadherin binding, sarcomere formation, and granule secretion was observed. Our findings demonstrated an association between ICUAW genes and sepsis-related mortality in the early phase of sepsis. Finally, defects in energy metabolism and muscle fiber formation are likely resulting in septic patients who are non-survivors, especially in respiratory-triggered sepsis.

Table of Contents

Introduction 1

Materials and methods 2

Results 4

Discussion 5

Reference 8

Table 1 11

Figures 12

About this Master's Thesis

Rights statement
  • Permission granted by the author to include this thesis or dissertation in this repository. All rights reserved by the author. Please contact the author for information regarding the reproduction and use of this thesis or dissertation.
School
Department
Subfield / Discipline
Degree
Submission
Language
  • English
Research Field
Keyword
Committee Chair / Thesis Advisor
Committee Members
Last modified

Primary PDF

Supplemental Files