Memory Assessment in a Nonhuman Primate Model of Alzheimer's Disease Using a Visual Paired-Comparison Task Pubblico
Martin, Ryan (2017)
Abstract
Importance: There is currently no animal model for Alzheimer's disease that fully recapitulates the etiologic, cognitive and pathological characteristics of the disease.
Objective: To determine if there is a transgenic nonhuman primate model of AD (AD monkey) recapitulates memory deficits present in human AD.
Measures: Visual Paired-Comparison task and quantitative PCR.
Subjects: The experimental group consists of two female AD monkeys aged between 9 and 10 years that contain the Swedish and Indiana mutations in βAPP under the control of human polyubiquitin promoter (APPswe/ind). Six macaques (3 males and 3 females) aged between 5 and 6 years were used as control. For molecular analysis, two female macaques aged between 9 and 10 years were used.
Results: Transgenic macaques displayed a significantly lower novelty preference on the Object-in-Place VPC task. All other differences in VPC tasks were not statistically significant. Up-regulation of the APP transcript in lymphocytes was observed in both AD monkeys. Down-regulation of ApoE, CX3CR1, and CXCL8 transcripts was observed in both AD monkeys.
Conclusion: AD monkeys show altered looking behavior on spatial-relational memory tasks, preferring familiar to novel stimuli. This altered looking behavior may be due to the increased expression of APP. A VPC task utilizing more visually similar stimuli than the VPC Color-Delay is required to determine if recognition memory is impaired.
Table of Contents
Introduction…………………………………………………………………………………….... 1
Methods……………………………………………………………….……………………....…..6
Figure 1: Stimuli present in VPC task variations…………….......……………...………13
Results……………………………………………………………………………...……………14
Figure 2: Color-Delay task…………………………………………...………………….18
Figure 3: Spatial-Location………....…………………………………………..………...19
Figure 4: Object-Replace………....……………………………...…………........……....20
Figure 5: Object-in-Place………....……………………….…………...……...………....21
Figure 6: Normalized RNA Lymphocyte Expression…………………......……………..22
Discussion……………………………………………………...………………………………...22
References………………………………………………………...……………………………...29
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