The effect of cystic fibrosis environment on the bactericidal and phagocytic abilities of macrophages Öffentlichkeit
Shenep, Melissa Anne (2012)
Abstract
Abstract
Alveolar cystic fibrosis (CF) macrophages have decreased innate
bactericidal abilities. Compared to non-CF macrophages in the
airway, CF macrophages are surrounded with an overabundance of
cytokines and a thick layer of dehydrated mucous. To test the
effect of the CF environment on the bactericidal and phagocytic
ability of the macrophage, we conducted in vitro
bactericidal assays. We infected mouse leukaemic monocyte
macrophage cell line (RAW 264.7) macrophages with Pseudomonas
aeruginosa (PA01) after conditioning the cells with CF plasma,
non-CF plasma, tumor necrosis factor α (TNFα),
interleukin-8 (IL-8), or CF sputum. Additionally, we performed an
in vivo bactericidal test in which we infected CF and
wild-type mice with PA01 and measured bacterial proliferation in
the bronchoalveolar fluid (BAF) and lungs. Furthermore, we tested
the respiratory burst of primary CF and non-CF monocytes, measured
the level of pro-inflammatory cytokines TNFα and IL-6 released
by primary CF monocytes after infection with PA01, and observed the
internalization of PA01 by CF macrophages. Although the CF
monocytes appeared to be able to internalize bacteria, produce
reactive oxygen species, and signal other cells via cytokines or
chemokines, they exhibited decreased bactericidal abilities when
tested in vivo or with TNFα or IL-8. Thus, we conclude
that the inflammatory nature of the CF environment inhibits the
bactericidal abilities of macrophages.
Table of Contents
Table of Contents
Introduction……………………………………………………...1
Materials and
Methods…………………………………........5
Figure 1
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Figure 2
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Figure 3
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Results……………………………………………………………..13
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Discussion……………………………………………...…..……..21
Future
Directions………………………………….…………….23
References.………………………………………..…………..….25
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