Effects of Aging and Enriched Environment On 5-Hydroxymethylcytosine (5-hmC) Levels in Mice Pubblico
Cai, Catherine Wen (2012)
Abstract
The study of epigenetics has gained particular attention and
interest in recent years as increasing numbers of studies have
demonstrated the importance of epigenetic regulation in governing
normal processes as well as its involvement in various disease
states. Epigenetic changes concern modifications to the genome that
influence gene expression in a cell- or tissue-specific manner, but
do not change the specific base sequence of the DNA. Recently, it
was discovered that the common epigenetic marker 5-methylcytosine
(5-mC) can be further modified by the Tet family of enzymes to
yield 5-hydroxymethylcytosine (5-hmC). In this study, we
characterize 5-hmC as a novel epigenetic mark and examine the
effect of exposure to an enriched environment, and more
specifically the effect of voluntary exercise, on levels of 5-hm in
various tissues. In previous experiments performed in our lab, it
was discovered that the levels of 5-hmC are higher in younger
animals and decrease as a result of exposure to an enriched
environment (Irier HA and Jin P., 2012). In this study, we explore
the effects of an enriched environment on the 5-hmC levels of
tissue from other major organs, including the heart and the lungs.
The study found that the heart and lung tissue share the brain's
age-related decline in 5-hmC levels, but in contrast with the
brain, exposure to an enriched environment increases the level of
5-hmC in the heart tissue.
Table of Contents
Introduction...1 to 5
Methods...6 to 12
Results...13 to 15
Discussion...16 to 19
Figures...20 to 25
References...26 to 28
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