Rare-earth doped apatite nanocrystals for cell and implanted biomaterial tracking Público

Abstract

Nano-sized apatite crystals are good candidates for repair or reconstruction of human bones and teeth, and main component of bone tissue engineering scaffolds, due to their excellent biocompatibility and osteogenicity. Apatite has a stable crystal structure, which could be employed as a host matrix for lanthanides doping to prepare both downconversion and upconversion fluorescent nanoparticles. In design of the thesis, the lanthanide doped apatite nanocrystals will be prepared by hydrothermal synthesis, and their structure, luminescence properties, cell proliferation, and fluorescent tracking will be investigated. The new fluorescent nano-apatite can not only form bonding with bone tissue, but also provide stable and clear fluorescence imaging. The novel fluorescence probe is expected to overcome the shortcomings such as instability and photobleaching of organic fluorophores, and the potential toxicity from quantum dots. They can be utilized for long-term bioimaging, distinguishing scaffold from newly formed bone tissue or identifying the interface between implanted material and bone tissue on the histological sections, and tracking the distribution of degraded scaffold fragments in vivo.

Table of Contents

TABLE OF CONTENTS

Page

CHAPTER 1 INTRODUCTION.. 1

Overview aims. 1

Background. 1

Selecting right doping matrix and dopants. 6

Research objective. 11

CHAPTER 2 Comparative investigation on crystal structure and cell behavior of rare-earth doped DOWNCONVERSION fluorescent apatite nanocrystals. 13

Introduction. 13

Materials and methods. 14

Results and discussion. 16

Conclusion. 32

CHAPTER 3 Investigation on structure and upconversion fluorescence of Yb³⁺/Ho³⁺ co-doped fluorapatite crystals for potential biomedical application.. 34

Introduction. 34

Materials and methods. 36

Results and discussion. 38

Conclusion. 57

CHAPTER 4 Lanthanides doped upconversion nanoapatite for smart bioimaging during bone regeneration.. 58

Introduction. 58

Materials and methods. 60

Results and discussion. 64

Conclusion. 76

CHAPTER 5 Conclusion AND OUTLOOK.. 77

APPENDIX An Amorphous silica-coated PCL electrospun fibers with a core-sheath structure for bioactive and biodegradable scaffolds. 81

Introduction. 81

Materials and methods. 83

Results and Discussion. 87

Conclusion. 96

References. 98

PUBLICATIONS. 110

VITA.. 111

About this Dissertation

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School	Laney Graduate School
Department	Biomedical Engineering
Degree	PhD
Submission	Dissertation
Language	English
Research Field	Engineering, Biomedical
Palabra Clave	Nanoapatite Fluorescence Upconversion Bioimaging Crystal model Lanthanide doping
Committee Chair / Thesis Advisor	Chen, Haifeng, PKU Xia, Younan, GT
Committee Members	Xiaojie, Duan, PKU Ying , Luo, PKU Yingfang, Ao, PKU 3rd Hospital

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