The Design and Assembly of 1D and 2D Multicomponent Collagen Mimetic Peptide Systems 公开

Abstract

The design of 1D and 2D multicomponent “soft” materials offers significant advantages over their single component counterparts. Multicomponent systems represent a viable option for introducing greater functionality and control over their assembled structures. However, fabrication methods for constructing multicomponent assemblies remains a significant challenge. Herein, we report the design of a series of collagen mimetic peptides (CMPs) that assemble into multicomponent ultra-large 1D tubes, having macroporous porosity, and 2D nanosheets. These assemblies comprise two or more peptides assembled via complementary electrostatic forces. We characterize their assembly using a suite of techniques including circular dichroism (CD) spectropolarimetry, transmission electron microscopy (TEM), dynamic light scattering (DLS), and atomic force microscopy (AFM). We demonstrate that by tuning the CMP sequences, we can tailor their assembly morphology and physical properties. Furthermore, we show that we can interconvert between 1D and 2D structures through pH changes of the assembly medium. These assemblies and, in particular, the 1D collagen tubes, represent an intriguing new addition into the biomolecular materials catalogue. The large physical dimensions of the tubes (pore size >100 nm) distinguishes these assemblies from other biomolecular 1D assemblies reported in literature. We envision that these assemblies may encapsulate large proteins or other biologically relevant molecules for applications in chemical sensing, catalysis, and as structural scaffolds for organizing nano- and mesoscale components. Altogether, these initial studies provide foundational design rules for assembling macroporous peptide-based tubes and nanosheets from multiple building blocks assembled through complementary coulombic interaction.

Table of Contents

Table of Contents

Introduction………………………………………………………………………………………1

Materials and Methods…………………………………………………………………………..12

            Materials…………………………………………………………………………………12

            Peptide Synthesis/Purification…………………………………………………………...12

            Circular Dichroism……………………………………………………………………….13

            Transmission Electron Microscopy……………………………………………………...14

            Zeta Potential Measurements…………………………………………………………….14

            Atomic Force Microscopy……………………………………………………………….15

            Stochastic Optical Reconstruction Microscopy………………………………………….15

Results and Discussion…………………………………………………………………………..16

Conclusion……………………………………………………………………………………….34

Supporting Information…………………………………………………………………………..37

References………………………………………………………………………………………..42

About this Honors Thesis

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School	Emory College
Department	Chemistry
Degree	B.S.
Submission	Honors Thesis
Language	English
Research Field	Chemistry, Biochemistry Nanoscience
关键词	1D Macroporous 2D Biomaterials Nanoscience Peptide Collagen
Committee Chair / Thesis Advisor	Vincent Conticello, Emory University
Committee Members	Patrick Cafferty, Emory University Jose Soria, Emory University

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