Food Reward in Dopamine β-Hydroxylase Knockout Mice Público
McGee, Jennifer Marie-Juergensen (2010)
Abstract
While dopamine (DA) has been implicated as an essential
neurotransmitter for reward,
norepinephrine (NE) has largely been ignored. To examine the role
of NE in food
reward, we tested mice that have been genetically altered to lack
an enzyme necessary for
NE synthesis (dopamine β-hydroxylase; DBH-/-) using fixed
ratio (FR) responding for 14
mg food pellets. The results suggest that genetic depletion of DBH
produces effects on
responding for food that, in males, are highly dependent on the
ratio requirement of the
schedule. When the number of responses required were low, FR1-FR14,
male DBH-/-
mice responded significantly more than DBH+/- controls. However, as
the ratio
requirement increased to FR16 through FR30, there were no
differences between the two
genotypes. There were no differences in low or high ratio
requirements between
genotypes in females and a post hoc analysis demonstrated the only
significant difference
between female genotypes was at FR1. 24-hour food intake was also
measured and it
was determined that male DBH-/- consumed more food per body weight
than male
controls, but that female DBH-/- mice consumed less than controls.
At the behavioral
level, these results were interpreted as reflecting two factors:
the physiological need for
food intake, and the incentive value of the food. At the
neuroscience level, it is proposed
that the chronic lack of NE causing an upregulation of DA
receptors, resulting in at low
FR values.
Table of Contents
Food Reward in Dopamine β-Hydroxylase Knockout
Mice............................................................
1
Methods
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7
Animals
.......................................................................................................................................
7
Apparatus
....................................................................................................................................
8
Training
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8
Testing
........................................................................................................................................
9
Results
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9
Operant
Responding.....................................................................................................................
9
Food Intake
...............................................................................................................................
10
Sex Differences
..........................................................................................................................
11
Discussion
.....................................................................................................................................
12
References
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17
Figures…………………………………………………………………………………………….29
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