Interaction of the Intra-S Phase Checkpoint and the Cellular Oxidative Stress Response in Saccharomyces cerevisiae Pubblico
Morgan, Heather Christina (2010)
Abstract
Interaction of the Intra-S Phase Checkpoint and the Cellular Oxidative Stress Response in Saccharomyces cerevisiae
by
Heather C. Morgan
Abstract
The response to DNA damage in Saccharomyces cerevisiae
and other eukaryotic
cells is regulated by the DNA damage checkpoints, a group of
proteins that act to
maintain genomic stability. While mechanisms of checkpoint
activation are poorly
understood, it is possible that an increase in intracellular
reactive oxygen species (ROS)
that functions as a DNA damage signal is mediating responses that
promote checkpoint
activity. This study will address whether the transcription factor
Yap1, a key mediator of
the oxidative stress response in yeast, modulates checkpoint
responses at the intra-S
phase checkpoint when cells are subject to genotoxic/replication
stress.
Table of Contents
Table of Contents
BACKGROUND..................................................................................................................1
MATERIALS AND
METHODS................................................................................................9
RESULTS........................................................................................................................11
DISCUSSION...................................................................................................................17
FIGURES
1. Model of the Role of Mec1 in the Regulation of Ribonucleotide
Reductase................................23
2. Model of the Role of Yap1 in the DNA Damage
Response......................................................23
3. Model of the Role of Yap1 in the Oxidative Stress Response and
Intra-S phase
Checkpoint
Activation.......................................................................................................24
4. Rnr4 Induction by MMS and HU in Strains with or without
Yap1.............................................24
5. MMS-induced
Cytotoxicity.............................................................................................25
6. HU-induced
Cytotoxicity................................................................................................25
7. Rnr4 Induction by MMS and HU in BER-deficient Strains with
or without
Yap1..............................................................................................................26
8. Identifying and Analyzing Mec1 via Western
Analysis..........................................................26
TABLES
1. Strain
Genotypes.........................................................................................................27
2. Summary of Rnr4
Induction............................................................................................27
3. Yap Response
Elements................................................................................................28
FIGURE AND TABLE
LEGENDS.............................................................................................29
REFERENCES..................................................................................................................32
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