Affect of form deprivation on visual thresholds in nob mice Open Access
Hadigal, Satvik Ramiah (2011)
Published
Abstract
Abstract
Affect of form deprivation on visual thresholds in nob
mice
By Satvik Hadigal
Purpose: Form deprivation induces exaggerated eye growth that leads
to myopia.
This shift in refractive error may negatively impact visual
function. In this study,
visual function of WT mice was measured before and after form
deprivation.
These results were compared to nob mice which have a defect
in the retinal ON-
pathway to determine the role of ON pathway transmission in visual
acuity and
contrast sensitivity after form deprivation myopia.
Methods: Refractive development of eyes during form deprivation was
measured
using an IR photorefractor. Visual acuity and contrast sensitivity
were measured
using an optokinetic tracking device. Mouse eyes were form deprived
using
diffuser goggles mounted in frames attached to head pedestals.
Baseline refractions
and visual thresholds of mice were taken prior to goggling.
Nob mice were
followed for 2 weeks and WT mice were followed for 4 weeks.
Refractions and
visual threshold measurements were repeated at the final timepoint,
after myopia
development.
Reuslts: Nob mice had a higher myopic shift when compared to
WT mice at 2 and
4 weeks of form deprivation, respectively. Baseline visual acuity
and contrast
sensitivity of nob mice were lower than WT mice. Visual
acuity and contrast
sensitivity of WT mice did not significantly change after myopia
development;
however visual acuity and contrast sensitivity of nob mice
decreased significantly.
While the control eyes of WT mice had stable visual thresholds
between the two
time points, the control eyes of nob mice increased in visual
threshold.
Conclusion: Nob mice with defective ON pathways have
increased susceptibility
to form deprivation and an associated decrease in visual acuity and
contrast
sensitivity. A functional ON pathway is necessary in post-natal
retinal
development to achieve peak visual performance.
Table of Contents
Table of Contents
1. Introduction- pg. 1
1.1 Figure 1 - pg. 4
1.2 Figure 2 - pg. 5
1.3 Figure 3 - pg. 10
2. Materials and Methods- pg. 12
2.1 Figure 4 - pg. 16
3. Results- pg. 16
3.1 Figure 5 - pg. 17
3.2 Figure 6 - pg. 18
3.3 Figure 7 - pg. 19
3.4 Figure 8 - pg. 20
3.5 Figure 9 - pg. 21
3.6 Figure 10 - pg. 22
4. Dicsussion- pg. 23
5. References- pg.27
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