Affect of form deprivation on visual thresholds in nob mice
By Satvik Hadigal
Purpose: Form deprivation induces exaggerated eye growth that leads to myopia.
This shift in refractive error may negatively impact visual function. In this study,
visual function of WT mice was measured before and after form deprivation.
These results were compared to nob mice which have a defect in the retinal ON-
pathway to determine the role of ON pathway transmission in visual acuity and
contrast sensitivity after form deprivation myopia.
Methods: Refractive development of eyes during form deprivation was measured
using an IR photorefractor. Visual acuity and contrast sensitivity were measured
using an optokinetic tracking device. Mouse eyes were form deprived using
diffuser goggles mounted in frames attached to head pedestals. Baseline refractions
and visual thresholds of mice were taken prior to goggling. Nob mice were
followed for 2 weeks and WT mice were followed for 4 weeks. Refractions and
visual threshold measurements were repeated at the final timepoint, after myopia
Reuslts: Nob mice had a higher myopic shift when compared to WT mice at 2 and
4 weeks of form deprivation, respectively. Baseline visual acuity and contrast
sensitivity of nob mice were lower than WT mice. Visual acuity and contrast
sensitivity of WT mice did not significantly change after myopia development;
however visual acuity and contrast sensitivity of nob mice decreased significantly.
While the control eyes of WT mice had stable visual thresholds between the two
time points, the control eyes of nob mice increased in visual threshold.
Conclusion: Nob mice with defective ON pathways have increased susceptibility
to form deprivation and an associated decrease in visual acuity and contrast
sensitivity. A functional ON pathway is necessary in post-natal retinal
development to achieve peak visual performance.
Table of Contents
Table of Contents
1. Introduction- pg. 1
1.1 Figure 1 - pg. 4
1.2 Figure 2 - pg. 5
1.3 Figure 3 - pg. 10
2. Materials and Methods- pg. 12
2.1 Figure 4 - pg. 16
3. Results- pg. 16
3.1 Figure 5 - pg. 17
3.2 Figure 6 - pg. 18
3.3 Figure 7 - pg. 19
3.4 Figure 8 - pg. 20
3.5 Figure 9 - pg. 21
3.6 Figure 10 - pg. 22
4. Dicsussion- pg. 23
5. References- pg.27
About this Honors Thesis
|Committee Chair / Thesis Advisor|
|Affect of form deprivation on visual thresholds in nob mice ()||2018-08-28||