Exploring the Regulation of cen mRNA Localization to Centrosomes Restricted; Files Only

Lee, Jina (Spring 2022)

Permanent URL: https://etd.library.emory.edu/concern/etds/hh63sx27j?locale=en
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Abstract

The centrosome plays a crucial role in maintaining genomic stability. Aberrant centrosome activities can manifest in mitotic errors, developmental disorders, and are associated with poor cancer prognosis. The activities and functions of centrosomes are regulated by the pericentriolar material (PCM), and previous studies have identified many different mRNAs localizing to the PCM. Understanding the role and function of mRNA at the centrosome is a topic of active investigation. Work from our laboratory recently demonstrated the localization of centrocortin (cen) mRNA is required for mitotic fidelity. The disruption of cen mRNA localization causes errors in cell division. However, the specific mechanism underlying cen mRNA localization to and regulation of the centrosome is not clear. Here, we investigated which cis and trans factors contribute to the localization of cen mRNA to the centrosome. We hypothesize that the RNA-binding protein Egalitarian (Egl) links cen mRNA to the motor protein dynein to transport cen to the centrosomes. To test this hypothesis, we examined the trans- and cis-acting elements required for cen mRNA localization using cen truncations and egl mutant lines. Identification of the mechanisms underlying cen mRNA localization to the centrosome will ultimately allow us to deepen our understanding of mRNA localization and centrosome regulation, aspects of which may be deregulated in the disease setting.

Table of Contents

Chapter 1: Introduction..........................................................................................1

Chapter 2: Results..................................................................................................6

Chapter 3: Discussion...........................................................................................12

Chapter 3: Material and Experimental Procedures...................................................17

Chapter 4: References............................................................................................22

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