Analysis of Electromyographic Features of the MitoPark Mouse Forearm Restricted; Files Only

Abstract

The characteristic locomotor disturbances of Parkinson's disease (PD) include shuffling gait, short steps, and low walking velocity. In this study, we investigated features of walking in the transgenic MitoPark mouse model for PD. We assessed gait and electromyographic (EMG) patterns of the biceps and triceps during voluntary, unimpeded locomotion. When compared to control mice, MitoPark mice demonstrated shorter strides and shorter EMG bursts in the biceps and lower amplitude bursts in the triceps.

Table of Contents

1.

Introduction............................................................................................................1

1.1

Parkinson’s Disease in Humans..............................................................................1

1.2

Mouse Models for PD.............................................................................................2

1.2.1

6-OHDA............................................................................................................2

1.2.2

MitoPark..........................................................................................................2

2.

Materials and Methods.........................................................................................3

2.1

Animals..................................................................................................................3

2.2

Surgery..................................................................................................................3

2.3

Open-Field Setup...................................................................................................5

2.4

Recordings.............................................................................................................5

2.5

Video Tracing.........................................................................................................6

2.6

Gait Tracking..........................................................................................................7

2.7

EMG Preprocessing................................................................................................8

2.8

Evaluation of EMG Quality.....................................................................................9

2.9

Analysis Methods...................................................................................................10

3.

Results....................................................................................................................13

3.1

Gait.........................................................................................................................13

3.1.1

Time Between Steps.........................................................................................13

3.1.2

MitoPark Mouse Takes Fewer, Shorter Steps..................................................14

3.1.3

The MP Mouse Moves Slower than the WT Mouse........................................15

3.2

EMG Analysis.........................................................................................................16

3.2.1

Representative EMG for Biceps and Triceps Show Variations in Noise...........16

3.2.2

The MP EMG Burst is Shorter than WT Bursts for Biceps but Not Triceps.....20

3.2.3

MP Peak Burst Magnitude is Lower Than WT Peak Burst Magnitude............21

3.2.4

Late Peak Times for MP Bursts.......................................................................22

4.

Discussion.............................................................................................................24

4.1

Triceps Differ in Amplitude and Peak Time but Not Duration..............................24

4.2

Biceps Differ in Amplitude, Peak Time, and Duration...........................................24

4.3

Relation to Gait.....................................................................................................24

4.4

Limitations............................................................................................................25

4.5

Challenges.............................................................................................................25

4.6

Future Directions...................................................................................................26

5.

References.............................................................................................................27

About this Honors Thesis

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• Permission granted by the author to include this thesis or dissertation in this repository. All rights reserved by the author. Please contact the author for information regarding the reproduction and use of this thesis or dissertation.

School	Emory College
Department	Biology
Degree	B.S.
Submission	Honors Thesis
Language	English
Research Field	Biology, General Biology, Neuroscience
Parola chiave	movement EMG behavioral biology Parkinson's Disease MitoPark electromyography motor control mice
Committee Chair / Thesis Advisor	Dieter Jaeger, Emory University
Committee Members	Svjetlana Miocinovic, Emory University Miguel Reyes, Emory University

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