Neuroanatomical and electrophysiological characteristics of dorsal column stimulation in the adult mouse Pubblico
Kravitz, Mark (2017)
Abstract
In this study, I present anatomical and physiological
findings related to the composition of primary afferents in the
adult mouse dorsal column. Analyzing electron microscope images, I
manually counted unmyelinated and myelinated axons, calculated mean
axon diameters, and characterized distribution of these fibers
throughout the dorsal column. My findings demonstrate the presence
of both myelinated and unmyelinated fibers with diameters
consistent with A-beta, A-delta , and C fibers. Using in vitro
electrophysiology techniques in four adult mouse spinal cords, I
recorded and analyzed the recruitment of afferent fiber populations
in the L6 and S1 dorsal roots during medial dorsal column
stimulation at the T10 and L5 segmental levels in the presence of
high and low concentrations of calcium. My findings demonstrate the
recruitment of A-beta, A-delta, and C fibers as well as
calcium-dependent, synaptically mediated activity. This study is
significant in that it is one of the few of its kind to be done in
adult mouse whole spinal cord preparations, an important model of
the mammalian central nervous system. The findings presented in
this study add to an understudied field of basic neuroanatomy and
electrophysiology of the mechanism underlying dorsal column
stimulation, a neurostimulatory therapy currently used clinically
for chronic pain, but with potential uses in other neurological
disorders.
Table of Contents
Introduction and Background………………………………………………………..……………..………1
Objectives……………………………………………………………………………………………………………7
Materials and Methods………………………………………..……………………………………………...10
Results………………………………………………………………………………………………………………16
Discussion………………………………………………………………………………………………………….19
Conclusion…………………………………………………………………………………………………...…….22
References…………………………………………………………………………………………………………23
Tables and Figures…………………………………………………………………………………..…………28
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