Assessing Impacts of Strain and Bedding type of Mice on Ambulation in Circadian Rhythm Test by Applying Linear and non-Linear Mixed Model, Poisson Mixed Model and Zero-Inflated Poisson Mixed Model Público

Xiong, Niya (Spring 2019)

Permanent URL: https://etd.library.emory.edu/concern/etds/6t053g99m?locale=es
Published

Abstract

Introduction: Circadian rhythm is the 24h biological cycle to facilitate an organism for daily environmental changes. It influences a broad range of biological processes, including neuronal, metabolic, and behavioral function. Circadian rhythm disruption may lead to acute and chronic impacts in behavior, wellbeing and health. Healthy laboratory animals with regular circadian rhythm are very crucial as appropriate models for research. In this study, the main goal is to examine whether mice strain type or cage bedding type will influence circadian rhythm behavior.

Methods: Number of ambulations was utilized to quantify mice activity. Linear and non-linear mixed model, Poisson mixed model and zero-inflated Poisson mixed model were introduced to assess effects of strain type, bedding type, time on mice ambulations in three different time periods, 0 ~ 23 h, initial three hours and dark phase.

Conclusions: For entire data (23 hours) analysis, B6 mice had higher activity compare to 129 mice, while when placing in 1/8 inch corn cob bedding cage would increase mice ambulations in contrast to 1/4 inch. Trends of strain type was similar when analyzing initial three hours data, however, 1/8 inch corn cob bedding type would decrease mice activity in this case. Results of dark phase analysis was unlike that of 23 hours or initial three hours. B6 mice still had higher activity while bedding type is not a significant risk factor of mice ambulations.

Keywords: Circadian Rhythm, Strain Type, Bedding Type, Linear or non-linear Mixed Model, Poisson Mixed Model, Zero-Inflated Poisson Mixed Model

Table of Contents

1. Introduction............................................................................................................................................ 1

2. Methods & Materials ............................................................................................................................. 3

2.1 Animals and housing conditions ........................................................................................................... 3

2.2 Circadian Rhythm Test ......................................................................................................................... 3

2.3 Data Summary ...................................................................................................................................... 3

2.4 Statistical Analysis ............................................................................................................................... 4

2.4.1 Fitting Models for Cross-Sectional Data....................................................................................... 4

2.4.2 Fitting Models for 23 Hours Longitudinal Data ........................................................................... 5

2.4.3 Fitting Models for Initial 3 Hours Longitudinal Data ................................................................... 5

2.4.4 Fitting Models for Dark Phase Longitudinal Data ........................................................................ 5

2.5 Statistical Consideration ....................................................................................................................... 6

3. Results ................................................................................................................................................... 6

3.1 Cross-Sectional Data Analysis of the Association between Strain type, Bedding type, Time and Number of Ambulation ............................................................................................................................... 6

3.2 Longitudinal Data Analysis of the Association between Strain type, Bedding type, Time and Number of Ambulation (23 hours) ........................................................................................................................... 8

3.3 Longitudinal Data Analysis of the Association between Strain type, Bedding type, Time and Number of Ambulation (Initial three hours) ........................................................................................................... 11

3.4 Longitudinal Data Analysis of the Association between Strain type, Bedding type, Time and Number of Ambulation (Dark Phase, 8.5h ~20.5h) ................................................................................................ 13

4. Discussion ............................................................................................................................................ 15

5. Reference ............................................................................................................................................. 18

6. Tables and Figures ............................................................................................................................... 20

7. Supplement Tables and Figures ........................................................................................................... 32

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