Epigenome-wide Association of HbA1c among the Non-diabetic Twins Public
Wang, Lijin (Spring 2020)
Published
Abstract
Aim: Conducted an epigenome-wide association study to identify novel HbA1c-associated CpG sites in whole blood DNA.
Patients & methods: We performed an epigenome-wide association study of hyperglycemia among 258 non-diabetic male twins using linear mixed effect models. DNA Methylation at each cytosine-phosphate-guanine (CpG) sites as a function of HbA1c, adjusting for age, smoking, BMI and calculated proportion of peripheral blood leukocytes subtypes.
Results: Regression analysis showed that identified three CpG sites significantly associated with HbA1c according to within-twin effect after multiple testing correction (FDR less than 0.05), including cg00725722 (CCDC74B, p-value = 2.74 × 10-7), cg19142411(RAD52, p-value = 9.54× 10-8), cg00773483 (RNF150; p-value = 2.04 × 10-7). We also identified 25 CpG sites significantly associated (FDR<0.05) with HbA1C via between-twin effect (unshared association between co-twins).
Conclusion: This epigenome-wide study discovered significant association (FDR <0.05) between HbA1c and DNA methylation level in within-twin and between-twin effects. There were no DNA methylation sites significantly associated with HbA1C in both between-twin effect and within-twin effect after multiple testing correction, which suggested that for different HbA1C-associated DNA methylation sites, the epigenetic associations were mostly driven by either shared or unshared factors, but not both.
Table of Contents
Introduction -----------------------------1
Methods ---------------------------------3
Results -----------------------------------5
Discussion------------------------------- 6
Conclusion -------------------------------9
Reference --------------------------------11
Tables -----------------------------------14
Figures and Figure Legends -------------19
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