Mapping the Core Promoter Element of UOL Gene Encoded by Herpes Simplex Virus 公开

Premkumar, Ajay (2010)

Permanent URL:


The activity of a gene promoter directly influences the level of gene expression. The activity of a promoter is highly regulated on both the epigenetic and DNA sequence levels, and regulatory mechanisms can be very complex. In order to understand the regulation of expression for a particular gene, identifying the key promoter elements in the promoter of that gene is an important step. The UOL ("Upstream of Latency-associated-transcript") gene of the Herpes Simplex Virus 1 (HSV-1) is expressed in acute infections and as a late gene in infected neuronal cells after reactivation of the latent HSV-1. The goal of my research is to map the core active region, and the key promoter elements of the UOL promoter. In this paper, I describe the use of luciferase assays to determine localized promoter regions of interest for the UOL gene in various cell lines. Previous studies have mapped the UOL promoter to a certain region and our results indicate different subregions within that region that may show enhancing and silencing activity, respectively. These results provide a basis for future analyses that will help determine the specific transcription factors and their binding sites of the UOL promoter. These transcription factors and binding sites could be future drug targets against the HSV-1 reactivation virulent phenotype.

Table of Contents


Figure 1...3

Materials and Methods...6

Figure 2...7
Figure 3...8

Results and Discussion...12

Figure 4...13
Figure 5...14
Figure 6...16
Figure 7...17
Figure 8...17
Figure 9...17
Figure 10...18
Figure 11...19
Figure 12...19
Figure 13...20
Figure 14...22
Figure 15...23
Figure 16...24


Appendix A; Figures...30

About this Honors Thesis

Rights statement
  • Permission granted by the author to include this thesis or dissertation in this repository. All rights reserved by the author. Please contact the author for information regarding the reproduction and use of this thesis or dissertation.
  • English
Research field
Committee Chair / Thesis Advisor
Committee Members

Primary PDF

Supplemental Files