Neuron-specific cellular functions of Interleukin-9 in Alzheimer’s Disease 公开

Abstract

The deposition of beta-amyloid and tau initiate immune responses in the brain that lead to neuroinflammation in Alzheimer’s Disease (AD). Increased rates of pro-inflammatory and anti-inflammatory cytokines such as Interleukin-9 (IL-9) are observed in AD. Previously, our lab found African Americans with AD had a higher increase in Cerebrospinal Fluid (CSF) IL- 9 levels than those with Normal Cognition (NC), but this was not true for Caucasians. Using immunohistochemical analysis for brain IL-9, we found greater brain IL-9 levels also associated with AD in African Americans but not Caucasians. The direct mechanism by which IL-9 acts on brain specific neurons remains to be elucidated. Here, we aimed at studying the role of IL-9 in the transmission and modulation of neuronal signals in the brain by investigating differential IL-9 and IL-9 Receptors (IL-9R) expression in cases with and without AD. Using postmortem brain slides from older African Americans and Caucasians, we utilized an immunohistochemical staining method to stain for IL-9R. We also aimed to observe the differences in density of the IL-9 receptor with or without ongoing cell loss. For the first time, we successfully identified the presence of IL-9R on neuronal cells, localizing around the cell body. We also found an upregulation of IL-9R in AD in comparison to control cases, and increased levels of perivascular IL-9 in comparison to parenchymal IL-9 in Caucasian AD cases which was not true for African American AD cases. These results support the finding that IL-9 has AD specific effects on the brain, and may be a key cytokine leading to the observed racial differences in AD. 

Table of Contents

Introduction 1

Materials & Methods 5

Results 8

Discussions 12

References 14 

About this Honors Thesis

Rights statement

• Permission granted by the author to include this thesis or dissertation in this repository. All rights reserved by the author. Please contact the author for information regarding the reproduction and use of this thesis or dissertation.

School	Emory College
Department	Biology
Degree	B.S.
Submission	Honors Thesis
Language	English
Research Field	Biology, Neuroscience Health Sciences, Aging Health Sciences, Immunology
关键词	Interleukin-9 Neuroinflammation Alzheimer's Disease Aging
Committee Chair / Thesis Advisor	William Hu, Emory University
Committee Members	Sara Culpepper, Emory University Rachelle Spell, Emory University Nicole Gerardo, Emory University

最新修改

Primary PDF

Thumbnail	Title	Date Uploaded	Actions
	Neuron-specific cellular functions of Interleukin-9 in Alzheimer’s Disease ()	2020-04-08 15:41:34 -0400	Press to Select an action Download

Supplemental Files

Thumbnail	Title	Date Uploaded	Actions