Deoxy-Amine Nucleoside Polymers (dANPs) to Control Gene Expression Open Access

Chen, Yen-Fu (2013)

Permanent URL: https://etd.library.emory.edu/concern/etds/1c18dg36b?locale=en
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Abstract

This study aims to explore the de novo synthesis of deoxy-amine nucleoside polymers (dANPs) that function as antisense oligonucleotides. The synthetic route requires 8 steps starting from thymidine. Template-directed ligation will be used to polymerize monomers into chains that can be 32 base pairs long. To confirm the cellular uptake of dANPs, fluorescent labeling will be used to monitor its location within the cell. To achieve maximum yields in the synthesis, alternative pathways are tested to overcome low reactivity in certain steps.

Table of Contents

Table of Contents

Abstract

List of Figures

I. Introduction..............................................................................................................................................................1

II. Experimental...........................................................................................................................................................6

III. Results.................................................................................................................................................................16

IV. Discussion

Rationale for starting material and synthetic route...........................................................................................................18

Oxidation reactivity......................................................................................................................................................18

Template-directed ligation............................................................................................................................................20

Demonstration of cellular uptake and future experiments..................................................................................................21

V. Conclusion..............................................................................................................................................................22

VI. References............................................................................................................................................................24

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