Fiber Photometry to Assess the Activity Patterns of Supramammillary Nos1 Neurons During Sleep, Wakefulness and Spontaneous Behavior in Mice Open Access
Seo, Hyun Seong (Spring 2020)
Abstract
Recent research from our laboratory has shown that the caudal hypothalamus, specifically the supramammillary nucleus (SuM), is a key and necessary node of the ascending arousal system, and sleep-wake regulatory system. Prior research has shown that there are different cell types in the SuM; glutamate-releasing neurons in the SuM can drive wakefulness, while neuronal cells containing nitric oxide synthase (NOS) appear to be important for rapid eye movement (REM)-related cortical activity, and the GABA/glutamate-containing population targets the hippocampus, densely innervating the dentate gyrus and CA2. Thus, the SuM has at least three populations of apparently functionally distinct neurons. While our prior work has been based on manipulations of these cellular groups, we have not examined the spontaneous activity of these neuronal groups during various states of arousal. To achieve this, we use the technique known as fiber photometry, permitting the real-time assay of calcium fluxes, as a proxy for neural activity, in genetically-targeted SuM neuronal groups. We will then correlate this activity with different behaviors and sleep-wake state changes in order to understand sleep-wake related activity in the different cellular populations within the SuM.
Table of Contents
Table of Contents
Introduction……………………………………………………………………………………..…1
Materials and Methods...……………………………………………………………………..……8
Results……………………………………………………………………………………………12
Discussion………………………………………………………………………………………..14
Future Directions...………………………………………………………………………………15
Technical Difficulties……………………………………………………………………………16
Appendix…………………………………………………………………………………………18
Figure 1…………………………………………………………………………………………..23
Figure 2…………………………………………………………………………………………..24
Figure 3…………………………………………………………………………………………..25
Figure 4…………………………………………………………………………………………..26
Figure 5…………………………………………………………………………………………..27
Figure 6…………………………………………………………………………………………..28
Figure 7…………………………………………………………………………………………..29
Figure 8…………………………………………………………………………………………..30
Figure 9…………………………………………………………………………………………..31
Figure 10…………………………………………………………………………………………32
Figure 11…………………………………………………………………………………………33
Figure 12…………………………………………………………………………………………34
Figure 13…………………………………………………………………………………………35
References...…………………………………………………………………………………..….36
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